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Diya Arora
Independent Researcher
India
Abstract
Bioethanol production from agricultural waste through fermentation offers a sustainable alternative to fossil fuels by valorizing lignocellulosic biomass. This study investigates process parameters affecting ethanol yield from rice straw and sugarcane bagasse using Saccharomyces cerevisiae. Pretreatment via dilute acid hydrolysis, enzymatic saccharification, and batch fermentation were optimized. A statistical analysis of key parameters (substrate loading, enzyme dose, fermentation time) is presented. Simulation of the fermentation kinetics is conducted using Monod-based models and validated experimentally. Results indicate a maximum ethanol concentration of 22.5 g/L and an overall yield of 0.45 g/g under optimized conditions. Five research objectives guide the work: (1) evaluate pretreatment efficacy, (2) quantify saccharification efficiency, (3) optimize fermentation parameters, (4) develop a kinetic model, (5) assess techno-economic viability. Simulation closely matches experimental data (R² = 0.96). The study demonstrates feasibility of bioethanol production from agricultural residues using technologies extant up to 2014.
Keywords
bioethanol, agricultural waste, fermentation, dilute acid hydrolysis, Saccharomyces cerevisiae
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